Kctd15 Knockout mouse models
Kctd15, the mouse ortholog of human KCTD15, encodes a BTB and POZ domain protein that oligomerizes into pentamers and acts both as a Cullin3 E3 ligase adaptor and as a direct inhibitor of AP-2 transcription factors, a mechanism first defined in neural crest induction where KCTD15 restricts the crest domain at the neural plate border. In vertebrate development it also modulates Wnt and Nodal signaling, and in the hypothalamus it has been implicated in the melanocortin pathway, consistent with human GWAS signals near KCTD15 for body mass index and obesity risk. Related family members KCTD1 and KCTD15 share the AP-2 inhibitory activity, and the closely related KCTD12 and KCTD17 diverge functionally, so paralog-aware design matters. Conditional nulls with Wnt1-Cre address neural crest derivatives, while hypothalamic or Pomc-restricted deletion addresses energy balance, and Cullin3-binding-deficient knockins separate ligase-dependent from transcription factor-binding functions. Choose the allele that matches whether the endpoint is craniofacial patterning, adiposity, or transcriptional output.
Kctd15 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Kctd15 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Kctd15 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Kctd15-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 201674 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Kctd15 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Kctd15 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Kctd15 Knockout mouse models are available?
When Kctd15 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Kctd15 knockout embryonic lethal in mice?
It depends on background and allele design. Some Kctd15 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Kctd15 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Kctd15?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.