Nfkb1 Knockout mouse models
Nfkb1, the mouse ortholog of human NFKB1, encodes p105, which is co-translationally and post-translationally processed to the p50 DNA-binding subunit; the precursor contains a Rel homology domain plus a carboxy-terminal ankyrin repeat region that functions as its own inhibitor, sequestering RelA and c-Rel until stimulus-dependent IKK phosphorylation and betaTrCP-mediated degradation of the ankyrin segment release active dimers. Because p50 lacks a transactivation domain, p50 homodimers act as repressors at kappaB sites, so the gene contributes both activating and silencing functions, and p105 additionally scaffolds Map3k8 to link NF-kB and Erk signaling. Homozygous nulls are viable with defective B cell responses, impaired isotype switching, and heightened infection susceptibility, making germline alleles tractable. A nonprocessable p105 knockin separates precursor inhibitor function from p50 output, conditional alleles restrict loss to a lineage, and humanization supports reagent matching. Rela, Relb, c-Rel, and Nfkb2 overlap at target promoters, so compound genetics is often necessary.
A conventional Nfkb1 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Nfkb1 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Nfkb1 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Designing a Nfkb1 Knockout allele
Choose the genotype to match the human disease. NFKB1 haploinsufficiency is the most frequently identified monogenic cause of common variable immunodeficiency, so heterozygous animals, not homozygous nulls, are the allelic match, and their phenotype is mild enough that aging cohorts and deliberate immunization are needed to reveal it. Homozygous nulls remain viable and answer a different question about total pathway architecture. Delete the exons encoding the Rel homology domain and confirm no ankyrin repeat only product remains, since a free carboxy terminal fragment would keep sequestering RelA and c-Rel and would confuse loss of function with gain of inhibition. Immunize with a hapten carrier conjugate and measure class switched and affinity matured titers, enumerate marginal zone and B1 compartments, challenge with Streptococcus pneumoniae or Listeria monocytogenes, and follow aged cohorts for spontaneous inflammation and autoantibodies.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Nfkb1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Nfkb1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Nfkb1 Knockout mouse models are available?
When Nfkb1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Nfkb1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Nfkb1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Nfkb1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Nfkb1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.