Rictor Knockout mouse models
Defining mTOR complex 2, Rictor is the ortholog of human RICTOR and encodes a large scaffold that, with mSIN1 and mLST8, confers substrate specificity for the hydrophobic motifs of AKT Ser473, SGK1 and conventional PKC isoforms, alongside a turn motif phosphorylation that stabilizes nascent AGC kinases. Unlike mTORC1, the complex resists acute rapamycin but is disassembled by prolonged exposure, which makes genetic ablation the only clean way to separate mTORC2 from pharmacologically confounded mTORC1 readouts. Germline deletion is embryonic lethal at midgestation, so conditional nulls with tissue-restricted Cre drivers are required, and running matched Rptor conditional cohorts is the standard way to attribute a phenotype to one complex rather than to the kinase itself. No paralog substitutes, though the accessory subunits Protor-1 and Protor-2 tune substrate selection, so interpret residual SGK1 signaling carefully when the endpoint is renal sodium handling or metabolic control.
Global Rictor deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Rictor Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Rictor knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Rictor-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 205043 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Rictor Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Rictor allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Rictor Knockout mouse models are available?
When Rictor Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Rictor knockout embryonic lethal in mice?
It depends on background and allele design. Some Rictor germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Rictor animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Rictor?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.