Slc17a7 Knockin mouse models
Cortical and hippocampal glutamatergic terminals depend on Slc17a7, the mouse ortholog of human SLC17A7, encoding VGLUT1, the SLC17 family vesicular transporter that fills synaptic vesicles using the proton motive force generated by the vacuolar ATPase and that additionally recruits endophilin A1 through its C-terminal polyproline region to slow vesicle endocytosis and shape release probability during sustained activity. VGLUT1 defines telencephalic excitatory identity and is developmentally exchanged for VGLUT2 at many synapses. Homozygous nulls survive birth but decline sharply after the third postnatal week with blindness, ataxia, and death, reflecting that developmental switch, so conditional alleles are strongly preferred for adult circuit work. Knockin Cre or reporter alleles at the locus provide clean access to cortical excitatory populations. Because VGLUT2 partially substitutes at a subset of synapses during early development, interpret quantal size and vesicle filling phenotypes with that transient overlap in mind and select region-specific Cre drivers accordingly.
Knockin designs at Slc17a7 support head to head comparisons between wild type and modified alleles because regulatory DNA stays in place.
What Slc17a7 Knockin mouse models are available?
ingenious targeting laboratory offers 4 distinct Slc17a7 knockin catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Slc17a7-IRES-Cre | Knockin | Cre/Dre Toolbox of Mice | live | KI 200086 | Inquire |
| Slc17a7-IRES-Flp | Knockin | Cre/Dre Toolbox of Mice | sperm cryopreservation | KI 241604 | Inquire |
| Slc17a7-2A-tdTomato | Knockin | fluorescent mouse | embryo cryopreservation | KI 210126 | Inquire |
| Slc17a7-2A-TagBFP | Knockin | fluorescent mouse | sperm cryopreservation | KI 220180 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Slc17a7 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Slc17a7 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Slc17a7 Knockin mouse models are available?
When Slc17a7 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Slc17a7 knockout embryonic lethal in mice?
It depends on background and allele design. Some Slc17a7 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Slc17a7 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Slc17a7?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.