Slc1a2 Knockin mouse models
Astrocytic clearance of synaptic glutamate is dominated by Slc1a2, the mouse ortholog of human SLC1A2, encoding GLT-1, also called EAAT2, a trimeric secondary active transporter in which each protomer combines a rigid scaffold domain with an elevator-like transport domain that carries glutamate across the membrane coupled to three sodium ions and one proton inward and one potassium ion outward, alongside an uncoupled chloride conductance. GLT-1 accounts for most forebrain glutamate uptake, its downregulation is a consistent feature of ALS and epilepsy, and dominant SLC1A2 variants cause developmental and epileptic encephalopathy 41. Global knockout mice develop lethal spontaneous seizures within weeks, so astrocyte-conditional deletion using GFAP-CreERT2 or Aldh1l1-CreERT2 is the practical route for adult studies. Neuronal GLT-1 exists but is minor, and compensation by Slc1a3 is partial at best; select patient missense knockins when dominant-negative trafficking rather than absence is the mechanism under test.
Knockin designs at Slc1a2 support head to head comparisons between wild type and modified alleles because regulatory DNA stays in place.
What Slc1a2 Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Slc1a2 knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Slc1a2-Luciferase-2A-CreERT2 | Knockin | Cre/Dre Toolbox of Mice, fluorescent mouse | sperm cryopreservation | KI 220304 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Slc1a2 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Slc1a2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Slc1a2 Knockin mouse models are available?
When Slc1a2 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Slc1a2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Slc1a2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Slc1a2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Slc1a2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.