Tardbp Knockout mouse models
Nuclear TDP-43, encoded by mouse Tardbp and orthologous to human TARDBP, is a heterogeneous nuclear ribonucleoprotein with two RRM domains binding UG-rich RNA and a C-terminal glycine-rich prion-like low complexity domain mediating phase separation and protein interaction. It represses cryptic exon inclusion in transcripts such as STMN2 and UNC13A, regulates splicing and mRNA stability, and autoregulates its own abundance by binding its 3-prime untranslated region. In ALS and frontotemporal lobar degeneration, TDP-43 mislocalizes from the nucleus and forms hyperphosphorylated, ubiquitinated cytoplasmic inclusions, and rare dominant mutations cluster in the low complexity domain, including M337V, A315T, and Q331K. Because Tardbp null embryos die around implantation and overexpression is independently toxic, endogenous locus knockins and conditional alleles are strongly preferred over transgenes. Match design to question, using nuclear localization signal mutants for mislocalization, patient missense knockins for aggregation, and humanized alleles when cryptic exon readouts require human sequence context.
Whole body loss of Tardbp gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Tardbp Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Tardbp (TDP43) knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Tardbp-KO | Knockout | KO/CKO mice, disease model mice | sperm cryopreservation | KO 210156 | Inquire |
Designing a Tardbp Knockout allele
A constitutive null has limited standalone value here: homozygotes die around implantation, and heterozygotes are close to normal because TDP-43 clamps its own abundance through 3-prime untranslated region binding, so protein rarely falls to half. That autoregulation makes the null a poor sensitizing background. Its real uses are genetic: place a patient missense or nuclear localization signal knockin over the null to remove the wild-type allele and ask whether the mutant supports viability and function, which distinguishes gain of function from haploinsufficiency more cleanly than homozygosing a knockin. Nulls also anchor antibody and reagent validation and provide null embryonic stem cells for chimera and complementation work. Delete the entire coding region rather than inserting a cassette into one exon, since cassette alleles frequently leak. Measure protein by quantitative western on tissue lysates, not transcript alone, and record embryonic lethality timing precisely.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Tardbp Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Tardbp allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Tardbp Knockout mouse models are available?
When Tardbp Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Tardbp knockout embryonic lethal in mice?
It depends on background and allele design. Some Tardbp germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Tardbp animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Tardbp?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.