Ticam1 Knockout mouse models
Encoding the adaptor TRIF, Ticam1 is the mouse ortholog of human TICAM1 and supplies the MyD88-independent arm of Toll-like receptor signaling, using a central TIR domain to engage TLR3 directly and TLR4 through TRAM (Ticam2) after endosomal internalization, an N-terminal region that recruits TRAF3 with the TBK1 and IKK epsilon complex for IRF3-driven type I interferon, and a C-terminal RHIM that binds RIPK1 and RIPK3 to trigger NF-kB, apoptosis, or necroptosis depending on caspase-8 availability. The classic Lps2 frameshift allele established that TRIF loss abolishes poly(I:C) responses and the late-phase LPS interferon response while sparing early MyD88 signaling. Nulls are viable, so germline deletion is tractable, whereas RHIM or TRAF-binding knockins separate interferon induction from death signaling and Myd88 compound alleles are needed because both adaptors serve TLR4. Fit alleles to interferon beta induction, endotoxin tolerance, or virus-induced tissue damage endpoints.
Whole body loss of Ticam1 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Ticam1 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Ticam1 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Ticam1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Ticam1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Ticam1 Knockout mouse models are available?
When Ticam1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Ticam1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Ticam1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Ticam1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Ticam1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.