Trp53 Conditional Knockout mouse — liver specific context
Trp53, the mouse ortholog of human TP53, encodes the tetrameric transcription factor whose central DNA-binding core reads p53 response elements at Cdkn1a, Mdm2, Bbc3, Pmaip1, and Zmat3, coupling ATM and ATR phosphorylation of the amino-terminal transactivation domains to arrest, senescence, and mitochondrial apoptosis while MDM2 and MDM4 impose continuous ubiquitin-dependent turnover. Because most human alleles are missense rather than deleted, the design fork is decisive: conditional or germline nulls model pure suppressor loss and yield thymic lymphoma and sarcoma, whereas structural and contact hotspot knockins such as R172H and R270H (human R175H and R273H) confer dominant-negative and gain-of-function metastatic behavior that a null never reproduces. Lox-stop-lox and restorable alleles interrogate reactivation therapeutics, and humanized TP53 supports MDM2 inhibitor and mutant-reactivator pharmacology. Note that Trp63 and Trp73 share response elements and can buffer apoptotic output, so match allele class to tumor spectrum, latency, and drug response endpoints deliberately.
Conditional deletion of Trp53 limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to Trp53 floxed stock yields usable cohorts. For liver work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Catalog options
Conditional knockout focuses the experiment on liver while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
| Model | Type | Category | Availability | Catalog # | |
|---|---|---|---|---|---|
| Trp53-Frox | Conditional Knockout | KO/CKO mice, disease model mice | sperm cryopreservation | CKO 190067 | Inquire |
| Trp53-Flox/Kras-LSL-G12D | Conditional Knockout | KO/CKO mice,point mutantion mice | live | CKO 233079 | Inquire |
| Trp53-Flox | Conditional Knockout | KO/CKO mice, disease model mice | live | CKO 18005 | Inquire |
Designing a Trp53 Conditional Knockout allele
Trp53 nulls survive to adulthood, so the argument for a conditional is not viability but attribution and timing: germline nulls succumb to thymic lymphoma by six months, which censors the slower epithelial tumors most projects actually want. Floxing exons 2 through 10 removes the transactivation domains and the entire DNA-binding core, giving a clean deletion rather than a truncated peptide that could retain tetramerization. Pair the allele to the compartment: Pdx1-Cre or p48-Cre with an LSL-Kras allele for pancreatic ductal disease, inhaled adenoviral Cre for lung, K14-Cre or WAP-Cre for mammary, GFAP-Cre for glioma. Cre itself provokes a p53-dependent damage response, so Cre-only littermates are not optional. Read out latency and spectrum, but also confirm function directly: irradiate sorted recombined cells and show failed Cdkn1a and Mdm2 induction alongside karyotypic instability.
Pricing and quotes
The Trp53 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Trp53 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with milestones for genotyping, QC, and dispatch.
FAQ
What Trp53 Conditional Knockout mouse models are available?
When Trp53 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Trp53 knockout embryonic lethal in mice?
It depends on background and allele design. Some Trp53 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for liver specific focused experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. Your note points to liver specific, so we start driver selection there.
Do you ship live Trp53 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Trp53?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.