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Recombination Systems & Tools

Cre-lox System

A bacteriophage P1-derived site-specific recombination technology that utilizes Cre recombinase to mediate genomic modifications at engineered LoxP sequences. This technology underpins conditional gene engineering approaches in mammalian genetics.

Overview

The Cre-lox system is a bacteriophage P1-derived site-specific recombination technology that utilizes Cre recombinase to mediate genomic modifications at engineered LoxP sequences. This technology underpins conditional gene engineering approaches that are foundational in contemporary mammalian genetics.

Frequently Asked Questions

What determines whether Cre-lox produces excision or inversion?

The relative orientation of LoxP sites: parallel orientation yields excision, while inverted orientation yields inversion.

Can Cre-lox be combined with other recombinases?

Yes. Cre-lox is often paired with Flp-FRT or Dre-rox for multi-layered control of complex genetic events.

Related Services

Related Terms

Floxed Gene / LoxP Site

A floxed gene contains two LoxP sites flanking an essential DNA segment, allowing Cre recombinase to mediate excision or inversion for conditional gene regulation. LoxP sites are 34 base pair sequences recognized by Cre recombinase.

Cre Driver Line (Tissue-Specific Cre)

A mouse line that expresses Cre recombinase from a promoter specific to certain tissues, cell types, or developmental stages, enabling targeted genomic recombination only in those cells.

Conditional Knockout (cKO) Mouse Models

A genetically engineered mouse in which a specific gene can be selectively inactivated in chosen tissues, cell types, or developmental stages—enabling precise studies of gene function while avoiding embryonic lethality or systemic effects.

More in Recombination Systems & Tools

Cre Driver Line (Tissue-Specific Cre)Floxed Gene / LoxP SiteLox-STOP-Lox (LSL) CassetteFlp-FRT SystemDre-rox System (Alternate Recombinase)
Cre Driver Line (Tissue-Specific Cre)View All TermsCytokine / Receptor Humanization

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