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Recombination Systems & Tools

Lox-STOP-Lox (LSL) Cassette

A cassette containing a transcriptional STOP sequence flanked by LoxP sites that blocks gene expression until excised by Cre recombinase. This molecular 'gatekeeper' design is essential for gain-of-function models requiring precise gene induction.

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Overview

An LSL cassette contains a transcriptional STOP sequence flanked by LoxP sites that blocks gene expression until excised by Cre recombinase. This molecular 'gatekeeper' design is essential for gain-of-function models requiring precise spatial and temporal gene induction.

Frequently Asked Questions

What is inside an LSL cassette?

STOP and polyA termination sequences, often accompanied by selectable markers, flanked by LoxP sites.

When should I use LSL designs?

For conditional gene activation or lineage tracing where spatial or temporal control is essential.

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Related Terms

Cre-lox System

A bacteriophage P1-derived site-specific recombination technology that utilizes Cre recombinase to mediate genomic modifications at engineered LoxP sequences. This technology underpins conditional gene engineering approaches in mammalian genetics.

Reporter Gene / Reporter Allele

A reporter gene is an easily detectable genetic marker—such as GFP, LacZ, or luciferase—inserted into a mouse genome to visualize gene expression, monitor cellular activity, or trace lineage in vivo.

Conditional Knockout (cKO) Mouse Models

A genetically engineered mouse in which a specific gene can be selectively inactivated in chosen tissues, cell types, or developmental stages—enabling precise studies of gene function while avoiding embryonic lethality or systemic effects.

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Large-Fragment / BAC TargetingView All TermsMissense and Nonsense Mutations

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