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Recombination Systems & Tools

Floxed Gene / LoxP Site

A floxed gene contains two LoxP sites flanking an essential DNA segment, allowing Cre recombinase to mediate excision or inversion for conditional gene regulation. LoxP sites are 34 base pair sequences recognized by Cre recombinase.

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Frequently Asked Questions

Where should LoxP sites be placed for a null allele?

Typically in introns flanking an essential exon to generate a nonfunctional transcript after excision.

How far apart can LoxP sites be?

Shorter distances favor efficient recombination; long segments may recombine less predictably.

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Related Terms

Cre-lox System

A bacteriophage P1-derived site-specific recombination technology that utilizes Cre recombinase to mediate genomic modifications at engineered LoxP sequences. This technology underpins conditional gene engineering approaches in mammalian genetics.

Conditional Knockout (cKO) Mouse Models

A genetically engineered mouse in which a specific gene can be selectively inactivated in chosen tissues, cell types, or developmental stages—enabling precise studies of gene function while avoiding embryonic lethality or systemic effects.

Lox-STOP-Lox (LSL) Cassette

A cassette containing a transcriptional STOP sequence flanked by LoxP sites that blocks gene expression until excised by Cre recombinase. This molecular 'gatekeeper' design is essential for gain-of-function models requiring precise gene induction.

More in Recombination Systems & Tools

Cre-lox SystemCre Driver Line (Tissue-Specific Cre)Lox-STOP-Lox (LSL) CassetteFlp-FRT SystemDre-rox System (Alternate Recombinase)
Dre-rox System (Alternate Recombinase)View All TermsFlp-FRT System

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