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Recombination Systems & Tools

Recombination Leakiness (Background Activity)

Unintended recombinase activity occurring without inducer presence or outside targeted tissues, leading to unwanted genomic alterations or mosaicism. Understanding its sources is essential for accurate genetic modeling.

Overview

Recombination leakiness refers to unintended recombinase activity occurring without inducer presence or outside targeted tissues, leading to unwanted genomic alterations or mosaicism. Understanding its sources and mitigation strategies is essential for accurate genetic modeling.

Frequently Asked Questions

What causes recombination without inducer?

Basal promoter activity, ligand-independent nuclear entry, or high transgene copy number.

How can I detect leakiness early?

Include Cre-reporter crosses and vehicle-only controls; quantify background recombination by PCR or imaging.

Related Services

Related Terms

Inducible Cre (Cre-ERT2 / Tamoxifen)

A system that fuses Cre recombinase to a modified estrogen-receptor ligand-binding domain (ERT2), confining it to the cytoplasm until activation by tamoxifen, thus providing temporal control of recombination.

Cre Driver Line (Tissue-Specific Cre)

A mouse line that expresses Cre recombinase from a promoter specific to certain tissues, cell types, or developmental stages, enabling targeted genomic recombination only in those cells.

Mosaicism (Genetic Mosaic)

The presence of two or more genetically distinct cell populations within the same organism that originated from a single fertilized egg. In genetic engineering, mosaicism arises when not all cells carry the intended genetic modification.

More in Recombination Systems & Tools

Cre-lox SystemCre Driver Line (Tissue-Specific Cre)Floxed Gene / LoxP SiteLox-STOP-Lox (LSL) CassetteFlp-FRT System
Pronuclear Injection (Transgenics)View All TermsReporter Gene / Reporter Allele

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