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Recombination Systems & Tools

Inducible Cre (Cre-ERT2 / Tamoxifen)

A system that fuses Cre recombinase to a modified estrogen-receptor ligand-binding domain (ERT2), confining it to the cytoplasm until activation by tamoxifen, thus providing temporal control of recombination.

Overview

Inducible Cre fuses Cre recombinase to a modified estrogen-receptor ligand-binding domain (ERT2), confining it to the cytoplasm until activation by tamoxifen. This provides temporal control of recombination for conditional genetic studies.

Frequently Asked Questions

How fast does recombination occur after tamoxifen?

Significant recombination typically appears within 24 to 72 hours, depending on tissue and dosage.

How do I reduce leakiness?

Use validated Cre-ERT2 lines, optimize dosing, and include no-tamoxifen controls with a reporter allele.

Related Services

Related Terms

Cre-lox System

A bacteriophage P1-derived site-specific recombination technology that utilizes Cre recombinase to mediate genomic modifications at engineered LoxP sequences. This technology underpins conditional gene engineering approaches in mammalian genetics.

Inducible Allele (Cre-ER Systems)

A genetically engineered version of a gene that can be activated or deleted at a specific time by an external stimulus. The most widely used approach is the Cre-ER system, allowing researchers to precisely control the timing of gene modification in vivo.

Temporal Control (Induction Windows, Dosing)

The activation or deactivation of a genetic modification at a specific time or during a defined developmental window. This strategy allows researchers to study gene function dynamically and distinguish developmental effects from later ones.

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