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Validation, QC & Genotyping

Zygosity (Heterozygous vs Homozygous)

Describes whether the two copies of a gene in an organism are identical (homozygous) or different (heterozygous). Determining zygosity is crucial for interpreting phenotypes and designing experiments.

Overview

Zygosity describes whether the two copies of a gene in an organism are identical (homozygous) or different (heterozygous). Determining zygosity is crucial for interpreting phenotypes and designing experiments involving genetically modified mice.

Frequently Asked Questions

Why does zygosity matter for phenotype?

Zygosity affects gene dosage, expression level, and resulting phenotype severity. Some phenotypes only manifest in homozygous animals.

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Related Terms

Allele / Genotype / Phenotype

An allele is a specific version of a gene, a genotype is the combination of alleles an organism carries, and a phenotype is the observable outcome of that genetic makeup interacting with environmental factors.

Breeding Scheme (Hetero × Hetero, etc.)

Planned mating strategies used to generate offspring of specific genotypes—such as heterozygous, homozygous, or compound mutant combinations—from established genetically modified lines.

Genotyping PCR / qPCR

Molecular methods used to detect, confirm, and quantify genetic modifications in engineered mouse models. These assays verify whether the targeted allele, wild-type allele, or transgene is present in an individual animal's DNA.

More in Validation, QC & Genotyping

Genotyping PCR / qPCRSouthern Blot / Copy Number ConfirmationSanger / NGS Validation of JunctionsOff-Target Assessment / Allele IntegrityGermline Confirmation / Coat Color Markers
Translational Relevance / Disease ModelingView All Terms

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