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Validation, QC & Genotyping

Genotyping PCR / qPCR

Molecular methods used to detect, confirm, and quantify genetic modifications in engineered mouse models. These assays verify whether the targeted allele, wild-type allele, or transgene is present in an individual animal's DNA.

Overview

Genotyping PCR and quantitative PCR (qPCR) are molecular methods used to detect, confirm, and quantify genetic modifications in engineered mouse models. These assays verify whether the targeted allele, wild-type allele, or transgene is present in an individual animal's DNA.

Frequently Asked Questions

What is the difference between PCR and qPCR genotyping?

Genotyping PCR identifies alleles based on PCR product size differences. qPCR provides quantitative analysis of gene copy number and zygosity.

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Related Terms

Southern Blot / Copy Number Confirmation

A classical DNA analysis method that remains the gold standard for verifying correct targeting, integration patterns, and copy number of inserted constructs in engineered mouse models.

Zygosity (Heterozygous vs Homozygous)

Describes whether the two copies of a gene in an organism are identical (homozygous) or different (heterozygous). Determining zygosity is crucial for interpreting phenotypes and designing experiments.

Breeding Scheme (Hetero × Hetero, etc.)

Planned mating strategies used to generate offspring of specific genotypes, such as heterozygous, homozygous, or compound mutant combinations, from established genetically modified lines.

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