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Validation, QC & Genotyping

Off-Target Assessment / Allele Integrity

Procedures to determine whether unintended genomic changes have occurred during editing, while also verifying that the targeted locus remains structurally sound and functionally unaffected.

Overview

Off-target assessment determines whether unintended genomic changes have occurred during editing, while allele integrity verifies that the targeted locus remains structurally sound and functionally unaffected.

Frequently Asked Questions

How are off-target effects detected?

Through in silico prediction, GUIDE-seq, or whole-genome sequencing (WGS) to identify potential off-target loci, combined with junction PCR and Southern blot to verify allele structure.

Related Services

Related Terms

Sanger / NGS Validation of Junctions

Sequencing tools for verifying the accuracy of DNA junctions at targeted integration sites. These assays confirm that homologous recombination or HDR occurred precisely without insertions, deletions, or rearrangements.

Southern Blot / Copy Number Confirmation

A classical DNA analysis method that remains the gold standard for verifying correct targeting, integration patterns, and copy number of inserted constructs in engineered mouse models.

Genotyping PCR / qPCR

Molecular methods used to detect, confirm, and quantify genetic modifications in engineered mouse models. These assays verify whether the targeted allele, wild-type allele, or transgene is present in an individual animal's DNA.

More in Validation, QC & Genotyping

Genotyping PCR / qPCRSouthern Blot / Copy Number ConfirmationSanger / NGS Validation of JunctionsGermline Confirmation / Coat Color MarkersBreeding Scheme (Hetero × Hetero, etc.)
Non-Homologous End Joining (NHEJ)View All TermsOpen Reading Frame (ORF)