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Validation, QC & Genotyping

Southern Blot / Copy Number Confirmation

A classical DNA analysis method that remains the gold standard for verifying correct targeting, integration patterns, and copy number of inserted constructs in engineered mouse models.

Overview

Southern blotting is a classical DNA analysis method that remains the gold standard for verifying correct targeting, integration patterns, and copy number of inserted constructs in engineered mouse models.

Frequently Asked Questions

Is Southern blotting still necessary with modern sequencing?

While modern tools like qPCR and NGS have supplemented Southern blots, the technique remains valuable for Pre Germline Validation before chimera generation due to its ability to detect both on-target and random integration events.

Related Services

Related Terms

Genotyping PCR / qPCR

Molecular methods used to detect, confirm, and quantify genetic modifications in engineered mouse models. These assays verify whether the targeted allele, wild-type allele, or transgene is present in an individual animal's DNA.

Sanger / NGS Validation of Junctions

Sequencing tools for verifying the accuracy of DNA junctions at targeted integration sites. These assays confirm that homologous recombination or HDR occurred precisely without insertions, deletions, or rearrangements.

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