Adipoq Knockout mouse models
Adipoq is the mouse ortholog of human ADIPOQ and encodes adiponectin, an adipocyte-secreted protein built from an amino-terminal collagen-like stalk and a carboxy-terminal C1q-like globular head that assembles into trimers, hexamers and high molecular weight oligomers, with the high molecular weight species carrying most insulin-sensitizing activity through Adipor1, Adipor2 and T-cadherin, activating AMPK and ceramidase pathways. Because bioactivity depends on oligomeric state rather than total protein, assays that measure only bulk levels can miss the relevant defect. Null mice show diet-induced insulin resistance with a modest chow phenotype, while knockins that block multimerization separate assembly from secretion, and humanized alleles support agonist and antibody pharmacology. Note that the widely used Adipoq-Cre driver is a knockin at this locus, so verify whether your line perturbs adiponectin dosage before attributing phenotypes to the floxed gene. Read out high molecular weight ELISA, hyperinsulinemic euglycemic clamp, and hepatic lipid content.
Adipoq null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Adipoq Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Adipoq knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Adipoq-KO(2) | Knockout | KO/CKO mice | live | KO 190438 | Inquire |
Designing a Adipoq Knockout allele
Constitutive deletion is a defensible primary allele, since homozygotes are viable and fertile with only mild chow phenotypes and the biology becomes visible under lipid load. Delete the entire coding region, both the collagen-like stalk exons and the globular head exons, because removing one domain can leave a secreted fragment that occupies receptors or distorts assembly of the remaining product. Interpret the allele against published discordance. Independently constructed nulls differ in the severity of diet-induced insulin resistance, and background plus deletion boundaries explain much of that, so state your strategy and use littermate controls on a fixed background. High fat feeding, lipid infusion, thiazolidinedione response, and crosses onto Apoe or Ldlr nulls for vascular endpoints are where the phenotype lives. Read glucose and insulin tolerance, free fatty acid clearance, tissue ceramide, and adipose macrophage infiltration rather than plasma protein alone.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Adipoq Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Adipoq allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Adipoq Knockout mouse models are available?
When Adipoq Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Adipoq knockout embryonic lethal in mice?
It depends on background and allele design. Some Adipoq germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Adipoq animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Adipoq?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.