Akt2 Knockout mouse models
Akt2 is the mouse ortholog of human AKT2 and encodes the insulin-responsive AKT isoform enriched in skeletal muscle, liver and adipose tissue, where PIP3 recruitment followed by PDK1 T309 and mTORC2 S474 phosphorylation licenses phosphorylation of AS160 and TBC1D1 to release GLUT4 vesicles, and of GSK3 beta and FOXO1 to suppress gluconeogenic transcription. Loss of function in humans produces severe insulin resistance with diabetes, and the corresponding mouse null recapitulates hyperglycemia, hyperinsulinemia and hepatic insulin resistance without embryonic lethality, making constitutive alleles usable while conditional alleles remain preferable for separating muscle, hepatocyte and adipocyte contributions. Activating E17K knockins model hypoinsulinemic hypoglycemia and provide a clean pharmacodynamic substrate for pan-AKT and isoform-selective inhibitors. Akt1 compensates broadly, so metabolic phenotypes sharpen considerably in compound heterozygous or double conditional backgrounds. Select nulls for metabolic dependency mapping, knockins for signaling gain, and tissue-restricted Cre lines matched to the insulin target organ under study.
Akt2 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Akt2 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Akt2 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Akt2-KO | Knockout | KO/CKO mice, disease model mice | sperm cryopreservation | KO 190742 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Akt2 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Akt2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Akt2 Knockout mouse models are available?
When Akt2 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Akt2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Akt2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Akt2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Akt2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.