Atr Knockout mouse models
Distinct from ATM in trigger and kinetics, Atr is the mouse ortholog of human ATR and encodes the PIKK that responds to RPA coated single stranded DNA at stalled forks and resected ends, recruited through its obligate partner ATRIP and activated allosterically by TOPBP1 or ETAA1 to phosphorylate CHK1 at S317 and S345, RPA32 and the 9-1-1 clamp, thereby suppressing origin firing, stabilizing forks and enforcing the replication checkpoint. Complete loss is cell lethal and embryonic lethal, so hypomorphic Seckel syndrome knockins and conditional floxed alleles with inducible Cre are the only workable designs for studying replication stress in vivo. Acute conditional deletion in adult tissue produces stem cell attrition and progeroid features, giving a mechanistic readout for ATR inhibitor toxicity. Chek1 sits immediately downstream and is not redundant, so do not expect ATM to substitute. Select hypomorphs for chronic replication stress biology and conditional nulls for acute dependency and inhibitor mechanism-of-action work in ATM deficient or oncogene driven tumors.
Global Atr deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Atr Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Atr knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Atr-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 205054 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Atr Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Atr allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Atr Knockout mouse models are available?
When Atr Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Atr knockout embryonic lethal in mice?
It depends on background and allele design. Some Atr germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Atr animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Atr?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Citations
- Altered phosphorylation of the proteasome subunit Rpt6 has minimal impact on synaptic plasticity and learning
- Sox17 Regulates a Program of Oligodendrocyte Progenitor Cell Expansion and Differentiation during Development and Repair
- The BRUCE-ATR Signaling Axis Is Required for Accurate DNA Replication and Suppression of Liver Cancer Development
- Deregulation of ZPR1 causes respiratory failure in spinal muscular atrophy
- Focal adhesion kinase activity is required for actomyosin contractility-based invasion of cells into dense 3D matrices.