Atrx Knockout mouse models
The X-linked Atrx locus, ortholog of human ATRX, encodes a SNF2-family ATPase whose ADD domain reads the combinatorial signal of unmethylated H3K4 with trimethylated H3K9, licensing recruitment to pericentromeric satellite repeats, telomeres, and imprinted loci. Acting as the obligate partner of DAXX, it deposits the replication-independent variant H3.3 into heterochromatin and resolves G-quadruplex structures during replication, so loss produces telomere dysfunction and the alternative lengthening of telomeres phenotype seen in pediatric glioblastoma, sarcoma, and pancreatic neuroendocrine tumors, alongside the developmental syndrome caused by hypomorphic missense alleles. Constitutive deletion is embryonic lethal, so exon-flanked conditional alleles combined with lineage Cre lines are standard, and hemizygous males versus heterozygous females demand explicit design decisions because of X inactivation mosaicism. No paralog substitutes for ADD-directed targeting. Select full conditional loss for telomere and genome instability endpoints, and point-mutant knockins when modeling syndromic missense biology.
Global Atrx deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Atrx Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Atrx knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Atrx-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 2104375 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Atrx Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Atrx allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Atrx Knockout mouse models are available?
When Atrx Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Atrx knockout embryonic lethal in mice?
It depends on background and allele design. Some Atrx germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Atrx animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Atrx?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.