Casp3 Knockin mouse models
Casp3 is the mouse ortholog of human CASP3 and encodes the principal executioner caspase, a short-prodomain zymogen activated by caspase-9 within the APAF1 apoptosome or by caspase-8 downstream of death receptors, then cleaving several hundred substrates including PARP1, the ICAD inhibitor of DFF40 whose removal unleashes internucleosomal DNA fragmentation, gelsolin, ROCK1 for membrane blebbing, and pannexin-1 for find-me signal release. Beyond death it mediates non-apoptotic events such as myoblast and osteoblast differentiation and dendritic spine pruning. Phenotype is strongly background dependent: on 129 the null shows exencephaly and perinatal lethality with excess neural progenitors, whereas on C57BL/6 mice are largely viable, so congenic state must be reported. Casp7 provides partial redundancy for a subset of substrates and compound Casp3 Casp7 alleles are needed for complete blockade. Use conditional flox designs with tissue Cre for lineage-restricted death questions, and catalytic mutant knockins when scaffold versus protease function is at issue.
An engineered Casp3 allele can introduce a human coding region, a point change, or a fluorescent reporter without random transgene integration noise.
What Casp3 Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Casp3 knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Casp3-IRES-YFP | Knockin | fluorescent mouse | sperm cryopreservation | KI 232076 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Casp3 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Casp3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Casp3 Knockin mouse models are available?
When Casp3 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Casp3 knockout embryonic lethal in mice?
It depends on background and allele design. Some Casp3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Casp3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Casp3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.