Ccne1 Knockout mouse models
Ccne1, mouse ortholog of human CCNE1, encodes cyclin E1, the late G1 partner of CDK2 whose activity peaks at the G1/S transition and drives phosphorylation of RB, p27, NPAT for histone gene transcription, and CDC6 and components of the prereplication complex to license origin firing and centrosome duplication. Cyclin E1 is destroyed by FBXW7 mediated ubiquitination after CDK2 autophosphorylation at threonine 380, and loss of that degron or 19q12 amplification produces constitutive S phase entry with rereplication, replication stress and chromosomal instability, a state now targeted therapeutically by PKMYT1 and WEE1 inhibitors in ovarian and endometrial cancer. Single nulls are viable, but Ccne1 and Ccne2 double nulls die in midgestation with failed trophoblast endoreplication, so redundancy is genuine and compound alleles are needed for requirement questions. Deploy conditional overexpression or degron-resistant T380A knockins for replication stress and synthetic lethality studies, and conditional nulls for endoreduplication and hepatocyte polyploidy endpoints.
A conventional Ccne1 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Ccne1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Ccne1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Ccne1-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 190931 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Ccne1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Ccne1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Ccne1 Knockout mouse models are available?
When Ccne1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Ccne1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Ccne1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Ccne1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Ccne1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.