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Cdh5 Conditional Knockout mouse models
Vascular endothelial cadherin is encoded by Cdh5, ortholog of human CDH5, a classical cadherin restricted to endothelium whose five extracellular repeats mediate calcium-dependent homophilic adhesion at adherens junctions while the cytoplasmic tail binds p120 catenin to block internalization and beta-catenin to link alpha-catenin and the actin cytoskeleton. VEGF-driven Src and Vav2 phosphorylation of specific tail tyrosines triggers junction disassembly and permeability, making this locus the molecular control point for vascular leak. Germline deletion is lethal near midgestation from failed vascular remodeling rather than failed vasculogenesis, so conditional alleles are mandatory, and phosphosite knockins that lock or mimic tyrosine modification separate adhesion from signaling far better than a null. Because Cdh5-CreERT2 is the field standard pan-endothelial driver, confirm that your floxed allele and driver are not competing at the same locus. Assess Evans blue extravasation, junction morphology by immunofluorescence, and retinal angiogenesis.
Conditional deletion of Cdh5 limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to Cdh5 floxed stock yields usable cohorts.
What Cdh5 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Cdh5 conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Cdh5-Flox | CKO | CKO | IN PRODUCTION | CKO 252571 | Inquire |
| These mice carry loxP sites flanking exon 3-4 of Cdh5 gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Cdh5 gene. | |||||
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Cdh5 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Cdh5 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Cdh5 Conditional Knockout mouse models are available?
When Cdh5 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Cdh5 knockout embryonic lethal in mice?
It depends on background and allele design. Some Cdh5 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Cdh5 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Cdh5?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ITL
Send the Cdh5 Conditional Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.