Cstb Knockout mouse models
Cstb is the mouse ortholog of human CSTB and encodes cystatin B (stefin B), a small monomeric cytosolic inhibitor of papain-family cysteine cathepsins that uses a wedge formed by its N-terminal glycine region and two hairpin loops to occlude the active sites of cathepsins B, L, S, and H, guarding against leaked lysosomal proteolysis. Loss produces oxidative stress, lysosomal instability, and cerebellar granule cell apoptosis, and mouse nulls develop progressive myoclonus, ataxia, and microglial activation reproducing human Unverricht-Lundborg disease, EPM1. Note a modeling constraint: most human EPM1 alleles are expansions of a dodecamer repeat in the CSTB promoter that reduce transcription, a mutation class that simple coding deletion cannot replicate, so promoter humanization or knockin of the repeat is required for expression-level fidelity. Related cystatins do not compensate in brain, so use the null for neurodegeneration endpoints and conditional alleles to test neuronal versus microglial origin.
A conventional Cstb knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Cstb Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Cstb knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Cstb-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 201501 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Cstb Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Cstb allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Cstb Knockout mouse models are available?
When Cstb Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Cstb knockout embryonic lethal in mice?
It depends on background and allele design. Some Cstb germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Cstb animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Cstb?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.