Dand5 Knockout mouse models
Dand5, the mouse ortholog of human DAND5 and also known as Cerl2 or Dante, encodes a secreted cysteine-knot DAN family antagonist that binds Nodal and restrains its activity specifically on the right side of the embryonic node, where leftward cilia-driven flow degrades its transcripts to break symmetry and permit left-sided Nodal, Lefty2, and Pitx2 expression. The gene therefore sits at the mechanotransduction to transcription interface of left-right patterning, downstream of Pkd2 and ciliary function and upstream of lateral plate asymmetry. Nulls are born with randomized cardiac looping, situs anomalies, and variable perinatal lethality, and human heterozygous variants segregate with laterality defects and congenital heart disease, so patient-matched missense knockins are often more faithful than complete deletion. Conditional alleles are less critical here than precise timing and node-restricted analysis, though floxed designs help dissect later cardiac roles. Related antagonists Cer1 and Lefty1 overlap partially, so compound genetics clarifies which Nodal pool is being controlled at each endpoint.
A conventional Dand5 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Dand5 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Dand5 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Dand5-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 230428 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Dand5 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Dand5 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Dand5 Knockout mouse models are available?
When Dand5 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Dand5 knockout embryonic lethal in mice?
It depends on background and allele design. Some Dand5 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Dand5 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Dand5?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.