Def Reporter Mouse
Since 1998, ingenious targeting laboratory has delivered 2,800+ projects with 100% germline transmission guarantee and 800+ peer reviewed publications.
Def is a common request for reporter mouse models. Ingenious targeting laboratory designs and delivers the line to your study specification with documented milestones.
Get a Quote in 24 HoursWhat Def Reporter mouse models are available?
ingenious targeting laboratory designs and generates Def reporter mouse models to your specification. Quotes return in about 24 hours with documented germline transmission and strict United States QC guidelines.
Scientific design
Def is a well studied locus in mouse genetics. Allele design follows your phenotype goals and what the published landscape already shows for this gene.
1 existing allele are indexed for Def. Where the published set lacks the modification you need, ingenious targeting laboratory builds that allele with documented germline transmission.
Reporter alleles fuse a detectable marker to endogenous regulation so expression mirrors the native locus. Pre germline characterization confirms signal location before germline transmission.
Quotes return in about 24 hours with milestones, pricing, and options for cryo or live dispatch.
Reporter options for Def
Def GFP reporter
Green fluorescent protein fused to Def regulatory elements reports native expression. Ingenious targeting laboratory engineers the knockin and confirms pattern before germline transmission.
Def YFP reporter
Yellow fluorescent protein fused to Def regulatory elements reports native expression. Ingenious targeting laboratory engineers the knockin and confirms pattern before germline transmission.
Def RFP reporter
Red fluorescent protein fused to Def regulatory elements reports native expression. Ingenious targeting laboratory engineers the knockin and confirms pattern before germline transmission.
Def mCherry reporter
Monomeric Cherry fluorescent protein fused to Def regulatory elements reports native expression. Ingenious targeting laboratory engineers the knockin and confirms pattern before germline transmission.
Def tdTomato reporter
Tandem dimer Tomato fused to Def regulatory elements reports native expression. Ingenious targeting laboratory engineers the knockin and confirms pattern before germline transmission.
Def lacZ reporter
Beta galactosidase reporter fused to Def regulatory elements reports native expression. Ingenious targeting laboratory engineers the knockin and confirms pattern before germline transmission.
Def Luciferase reporter
Bioluminescent reporter fused to Def regulatory elements reports native expression. Ingenious targeting laboratory engineers the knockin and confirms pattern before germline transmission.
What researchers say
“Ingenious targeting laboratory delivered exactly the floxed allele we designed, with clear milestones and germline confirmed founders.”
“The team translated our humanization requirements into a knockin strategy we could breed and phenotype on schedule.”
“Clear communication on allele design and germline transmission made our conditional knockout program straightforward to execute.”
Selected publications
- Ido1 and Ido2 deficiencies attenuate kainic acid-induced ictogenesis
- Spatial Mapping of CoQ10 Repletion by BPM31510 in a Genetic Mouse Model (Coq4F147C) of Coenzyme Q Deficiency
- Ido1 or Ido2 deficiency in myeloid-derived cells attenuates TMEV-induced ictogenesis
- Expression of mutant TIE2 p.L914F during mouse development causes embryonic lethality and defects in vascular remodeling
- Deficiency of factor-inhibiting HIF creates a tumor-promoting immune microenvironment
FAQ
What Def Reporter mouse models are available?
When Def Reporter lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Def knockout embryonic lethal in mice?
It depends on background and allele design. Some Def germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Def animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Def?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.