Erbb3 Knockout mouse models
Erbb3, the mouse ortholog of human ERBB3, encodes a pseudokinase family member that binds neuregulin 1 and neuregulin 2 through extracellular domains I and III but carries substitutions in the catalytic loop that render it essentially kinase dead, so it functions as an allosteric activator of a partner kinase and, critically, as the ERBB family member with six YXXM motifs in its C-terminal tail that directly recruit the p85 subunit of PI3K. This makes ERBB3 the dominant conduit to AKT for ERBB2 amplified tumors and the principal mechanism of adaptive resistance to EGFR and ERBB2 targeted agents, since relief of feedback rapidly upregulates ERBB3. Null embryos die at midgestation with cardiac cushion and Schwann cell precursor defects, so conditional alleles are needed for adult work. Humanized ectodomain knockins support testing of ERBB3 antibodies and bispecifics whose epitopes do not cross-react. Choose conditional nulls for PI3K dependency and resistance studies, paired with an epithelial or Schwann cell Cre.
A conventional Erbb3 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Erbb3 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Erbb3 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Erbb3-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 234965 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Erbb3 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Erbb3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Erbb3 Knockout mouse models are available?
When Erbb3 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Erbb3 knockout embryonic lethal in mice?
It depends on background and allele design. Some Erbb3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Erbb3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Erbb3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.