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Foxp3 Knockout mouse models
Foxp3, the mouse ortholog of human FOXP3, encodes a forkhead-box transcription factor whose FKH DNA-binding domain, leucine zipper dimerization region, and N-terminal repressor module nucleate large complexes with Runx1/CBFβ, NFAT, Eos, and the NuRD and CoREST corepressors to enforce the regulatory T cell program, repressing Il2 while sustaining Il2ra, Ctla4, and Ikzf2. Lineage specification requires sustained TCR signaling plus demethylation of conserved noncoding sequence 2, which locks heritable expression; loss produces the scurfy phenotype, an X-linked fatal lymphoproliferative wasting syndrome in hemizygous males that mirrors human IPEX. Design decisions follow directly. Null and conditional alleles ask whether Treg identity is required in a given tissue or temporal window, Cre and reporter knockins (including inducible Foxp3-CreERT2 for fate mapping or diphtheria-toxin ablation) resolve lineage stability, and IPEX point-mutation or humanized alleles interrogate patient variants. No paralog substitutes for Foxp3, so deletion timing and driver fidelity, not redundancy, dominate interpretation.
A conventional Foxp3 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Foxp3 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Foxp3 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Foxp3-KO(2) | KO | KO | DEVELOPING | KO 263146 | Inquire |
| The exon 4-8 of Foxp3 gene was deleted to generate Foxp3 knockout mouse. | |||||
Designing a Foxp3 Knockout allele
A germline null is not a tool with a long working window; hemizygous males develop lymphoproliferative wasting, scaly ears and tails, and hepatosplenomegaly, and die within roughly the first month, so every experiment must fit inside that interval. Colony management follows. Maintain through heterozygous females, genotype sex and allele together, and expect heterozygous females to stay largely healthy because wild type regulatory T cells outcompete deleted ones. Delete the promoter with the first coding exon, or the entire coding region, rather than an internal exon that could yield an amino terminal repressor fragment. Use this allele for rescue studies, asking whether transfer of wild type or engineered regulatory cells prevents disease, and for bone marrow chimeras. Endpoints are survival curves, weight, infiltrates in skin, lung, liver and pancreas, activated T cell frequencies, and autoantibody titers.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Foxp3 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Foxp3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Foxp3 Knockout mouse models are available?
When Foxp3 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Foxp3 knockout embryonic lethal in mice?
It depends on background and allele design. Some Foxp3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Foxp3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Foxp3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ingenious targeting laboratory
Send the Foxp3 Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.