Gapdh Knockin mouse models
Cytosolic NAD+ turnover is set by Gapdh, the mouse ortholog of human GAPDH, which encodes the homotetrameric glyceraldehyde-3-phosphate dehydrogenase that oxidizes and phosphorylates glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate while reducing NAD+, the step that gates the payoff phase of glycolysis and feeds substrate-level phosphorylation. Its active site cysteine makes the enzyme a redox sensor, since oxidation or S-nitrosylation redirects the protein toward nuclear functions including SIAH1-dependent degradation cascades, OCA-S-linked histone gene transcription and mRNA binding, and the same residue underlies inhibition by koningic acid and adduction by methylglyoxal. Because glycolytic flux is essential in nearly every lineage, germline deletion is not a viable design, and even partial manipulation perturbs the housekeeping standard commonly used for normalization. Practical alleles are conditional nulls with tamoxifen-inducible Cre for acute flux collapse, active site cysteine knockins to separate moonlighting from catalysis, and epitope-tagged or humanized knockins for pharmacology. Align allele with endpoint, whether extracellular acidification, NAD+ to NADH ratio, or in vivo glucose tracing.
Knockin designs at Gapdh support head to head comparisons between wild type and modified alleles because regulatory DNA stays in place.
What Gapdh Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Gapdh knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Gapdh-IRES-mTagBFP2 | Knockin | fluorescent mouse | sperm cryopreservation | KI 200031 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Gapdh Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Gapdh allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Gapdh Knockin mouse models are available?
When Gapdh Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Gapdh knockout embryonic lethal in mice?
It depends on background and allele design. Some Gapdh germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Gapdh animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Gapdh?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.