Glp1r Knockout mouse models
Glp1r is the mouse ortholog of human GLP1R and encodes a class B1 secretin-family G protein-coupled receptor that engages GLP-1 by a two-domain mechanism, with the large extracellular domain capturing the peptide carboxy terminus before the peptide amino terminus inserts into the transmembrane bundle to trigger Gs coupling, cyclic AMP production and PKA and Epac2 signaling that potentiates glucose-dependent insulin secretion. Beyond beta cells, hindbrain and hypothalamic receptor populations mediate satiety and gastric emptying, so circuit-level and islet-level questions demand different tools. Because incretin mimetics including semaglutide were optimized against the human receptor and species differences in the extracellular domain alter potency, humanized GLP1R knockins are the honest platform for pharmacology, while conditional nulls with a validated tissue-restricted or neuron-specific Cre assign efficacy to a compartment. Glp1r-Cre lines are knockins, so check dosage. Endpoints include glucose tolerance, insulin secretion, food intake and body weight.
Glp1r null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Glp1r Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Glp1r knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Glp1r-KO | Knockout | KO/CKO mice, disease model mice | live | KO 190803 | Inquire |
Designing a Glp1r Knockout allele
The strongest use of a straight null is as a specificity control. Homozygotes are viable with mild fasting hyperglycemia and blunted glucose-stimulated insulin secretion, and their complete failure to respond to exenatide, liraglutide, or semaglutide is what proves an in vivo effect is receptor mediated rather than off target, including the weight loss and gastric emptying components. Delete across the coding exons rather than the promoter, and confirm absence of protein by radioligand binding or a validated antibody, since transcript-based checks are unreliable at low abundance. Interpretation requires two cautions. Lifelong absence permits compensation, notably increased reliance on Gipr, so pair with Gipr nulls or double receptor mutants when the incretin axis is the subject, and developmental effects on islet architecture and ventricular wall thickness confound adult readouts. Score oral and intraperitoneal glucose tolerance, insulin secretion, gastric emptying, and agonist nonresponsiveness.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Glp1r Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Glp1r allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Glp1r Knockout mouse models are available?
When Glp1r Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Glp1r knockout embryonic lethal in mice?
It depends on background and allele design. Some Glp1r germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Glp1r animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Glp1r?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.