H2 Conditional Knockout mouse — Ins2-Cre pairing
Conditional deletion of H2 limits genetic change to the lineage you choose. That precision matters for oncology, immunology, and metabolic work where systemic loss would confound interpretation. After you confirm your Cre specificity, crossing to H2 floxed stock yields interpretable cohorts. For pancreatic beta cell work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Driver pairing
Ins2-Cre biases toward pancreatic beta cell. Inducible: no.
Open Ins2-Cre hub page · pancreatic beta cell Cre line index
Catalog options
Conditional knockout keeps pancreas-beta as the experimental theater while the rest of the animal retains a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
| Model | Type | Category | Availability | Catalog # | |
|---|---|---|---|---|---|
| H2-Ab1-Flox | Conditional Knockout | KO/CKO mice | live | CKO 200183 | Inquire |
| H2-T23-Flox | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 220482 | Inquire |
| H2-Aa-Flox | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 240017 | Inquire |
| H2-K1-Flox | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 232188 | Inquire |
Why this approach
Conditional knockout keeps neuron as the experimental theater while the rest of the animal retains a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Build timeline and pricing
Expect roughly twenty six weeks to study ready animals for many standard conditional crosses after contract start. We return quotes in about twenty four hours and map milestones for genotyping, QC, and dispatch.
Open the request quote formFAQ
How long does a H2 conditional knockout project take?
Most custom conditional knockout projects run near twenty six weeks from contract activation to study ready animals when breeding is straightforward. Complex humanization or multi allele stacks can add time. We return detailed quotes within about twenty four hours so you can align cohort start dates with grant or IND milestones.
Is H2 knockout embryonic lethal in mice?
Lethality depends on genetic background and exact allele design. Some H2 germline knockouts are viable, others require conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null approaches before you commit.
Which Cre driver is best for ins2 cre focused experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak patterns. We map your organ and cell type to a short list of proven Cre lines, then discuss reporter crosses and controls. Your query highlights ins2 cre as a primary axis, which we treat as the starting point for driver selection.
Do you ship live H2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a custom project with cryo or live dispatch options depending on cohort timing and geography.
How do I request a quote for H2?
Use the catalog inquire buttons or the request quote form with your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.