Irak1 Knockout mouse models
Irak1, the mouse ortholog of human IRAK1 and an X-linked gene adjacent to Mecp2, encodes an interleukin 1 receptor associated kinase with an N-terminal death domain, a ProST region, and a kinase domain that is recruited into the MyD88 and IRAK4 Myddosome after TLR or IL-1R engagement. IRAK4 phosphorylates IRAK1, which then autophosphorylates, undergoes K63 linked ubiquitination, and activates TRAF6, TAK1, and the IKK complex, while in plasmacytoid dendritic cells IRAK1 additionally phosphorylates IRF7 to drive endosomal TLR7 and TLR9 dependent type I interferon. Because IRAK2 provides substantial redundancy for TRAF6 activation, Irak1 nulls show blunted rather than abolished NF-kB responses while the interferon defect is more penetrant, so interpret partial phenotypes accordingly and consider compound Irak2 alleles. Kinase-dead knockins separate catalytic from scaffolding roles, the relevant comparison for degrader versus inhibitor programs, and conditional deletion avoids confounding from this X-linked neighborhood.
A conventional Irak1 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Irak1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Irak1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Irak1-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 190298 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Irak1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Irak1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Irak1 Knockout mouse models are available?
When Irak1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Irak1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Irak1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Irak1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Irak1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.