Mapk3 Knockout mouse models
Mapk3, the mouse ortholog of human MAPK3, encodes ERK1, which shares activation by MEK1 and MEK2 through its TEY motif, the same D-domain and DEF-site substrate recognition surfaces, and essentially the same catalytic preferences as ERK2, differing mainly in an amino-terminal extension that slows its nuclear shuttling and in lower expression in most tissues. Homozygous nulls are viable and fertile with modest thymocyte maturation and adipogenic phenotypes, consistent with ERK2 supplying the bulk of pathway flux, which makes this locus most useful for dosage experiments rather than for asking whether ERK activity is required. Informative designs therefore include a straightforward null on sensitized backgrounds, conditional deletion combined with Mapk1 floxed alleles to titrate total ERK output, and isoform-swap knockins that place ERK1 coding sequence at the Mapk1 locus to test whether the two proteins are functionally interchangeable. Interpretation depends on quantifying phospho-ERK dosage, so pair allele choice with a driver validated for complete excision.
Mapk3 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Mapk3 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Mapk3 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Mapk3-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 191093 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Mapk3 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Mapk3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Mapk3 Knockout mouse models are available?
When Mapk3 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Mapk3 knockout embryonic lethal in mice?
It depends on background and allele design. Some Mapk3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Mapk3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Mapk3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.