Mapk8 Knockout mouse models
Encoding JNK1, Mapk8 is the mouse ortholog of human MAPK8 and is activated by Map2k4 and Map2k7 phosphorylation of its TPY motif downstream of Map3k proteins responding to cytokines, ultraviolet irradiation, and endoplasmic reticulum stress, with Jip scaffolds organizing the module; substrates include Jun at Ser63 and Ser73, Atf2, Jund, Bcl2l11, and Irs1 at Ser307, linking stress signaling to apoptosis, transformation, and insulin resistance, while alternative splicing generates alpha and beta isoforms with distinct substrate docking. Homozygous nulls are viable and metabolically protected on high fat diet, whereas Mapk8 with Mapk9 double nulls die in mid-gestation from dysregulated neural apoptosis, which is the clearest evidence that JNK1 and JNK2 are genuinely redundant and that single-gene results must be interpreted cautiously. Conditional alleles isolate tissue-autonomous metabolic roles, analog-sensitive mutants allow acute inhibition, and compound conditional genetics with a lineage-specific driver is usually necessary for developmental questions.
Whole body loss of Mapk8 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Mapk8 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Mapk8 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Mapk8-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 190836 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Mapk8 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Mapk8 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Mapk8 Knockout mouse models are available?
When Mapk8 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Mapk8 knockout embryonic lethal in mice?
It depends on background and allele design. Some Mapk8 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Mapk8 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Mapk8?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.