Ms4a1 Knockout mouse models
Ms4a1, ortholog of human MS4A1, encodes CD20, a four-pass transmembrane member of the MS4A family with no identified natural ligand that associates with the B cell receptor complex and modulates store-operated calcium entry, appearing from the late pre-B stage through memory B cells and disappearing upon plasma cell differentiation. The decisive design consideration is that the null is largely uninformative, since knockout mice retain near-normal B cell development with only subtle calcium signaling changes, while the clinically meaningful biology lives entirely in the large extracellular loop that rituximab, ofatumumab and obinutuzumab recognize and that differs between mouse and human. Epitope-matched humanized CD20 knockins are therefore mandatory for studying depletion kinetics, complement-dependent cytotoxicity, antibody-dependent phagocytosis and Fc receptor engagement. Related MS4A family members are co-expressed and may buffer signaling contributions. Read out B cell depletion depth and duration across tissues, complement consumption, and recovery from bone marrow progenitors.
A conventional Ms4a1 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Ms4a1 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Ms4a1 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Ms4a1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Ms4a1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Ms4a1 Knockout mouse models are available?
When Ms4a1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Ms4a1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Ms4a1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Ms4a1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Ms4a1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.