Myh6 Conditional Knockout mouse — Myh6-Cre pairing
Myh6, the mouse ortholog of human MYH6, encodes alpha myosin heavy chain, the fast ATPase cardiac motor whose isoform biology carries a species caveat that shapes every study: alpha predominates in the adult mouse ventricle whereas the human ventricle runs mainly on beta myosin from MYH7, so contractile kinetics and the pathological switch from alpha toward beta during hypertrophy and failure do not map directly onto patients. Human MYH6 variants cause atrial septal defects and cardiomyopathy, arguing for patient-specific knockins over deletion when modeling disease. A further practical warning applies because the Myh6 promoter drives the widely used alpha-MHC-Cre and MerCreMer cardiac lines, and tamoxifen-treated MerCreMer animals develop transient cardiomyopathy on their own, so Cre-only tamoxifen-treated controls are non-negotiable. Conditional alleles paired with echocardiography, sarcomere shortening in isolated myocytes, fetal gene program transcripts, and myosin ATPase measurements match allele class to a mechanistic endpoint.
A floxed Myh6 allele paired with Cre gives spatial control that whole body knockouts cannot offer. People use it to separate developmental roles from adult homeostasis, to model somatic mutations, and to match disease that begins in one organ. For cardiac work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Driver pairing
Myh6-Cre biases toward cardiac. Inducible: no.
Catalog options
Conditional knockout focuses the experiment on heart while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
| Model | Type | Category | Availability | Catalog # | |
|---|---|---|---|---|---|
| Myh6-Flox | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 242563 | Inquire |
Designing a Myh6 Conditional Knockout allele
Germline loss kills embryos during cardiac looping, so a floxed allele is the only route to adult phenotypes, and adult deletion is severe rather than subtle because this isoform carries most ventricular contraction in mouse. There is a design trap specific to this gene: the standard cardiac drivers are transcribed from the Myh6 promoter itself, and once cells lose alpha myosin and switch toward beta, that promoter is downregulated, so Cre falls off and unrecombined myocytes persist as escapers. Prefer a driver independent of the locus, such as Tnnt2 or Actn2 based inducible lines or AAV9 delivered cTnT-Cre, and always confirm recombination in isolated myocytes. Flank the promoter and initial coding exons rather than internal motor domain exons, since a truncated head fragment incorporates into thick filaments and acts dominantly. Quantify escaper fraction alongside ventricular geometry and isolated myocyte shortening.
Pricing and quotes
The Myh6 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Myh6 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with milestones for genotyping, QC, and dispatch.
FAQ
What Myh6 Conditional Knockout mouse models are available?
When Myh6 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Myh6 knockout embryonic lethal in mice?
It depends on background and allele design. Some Myh6 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for myh6 cre focused experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. Your note points to myh6 cre, so we start driver selection there.
Do you ship live Myh6 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Myh6?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.