Myh7 Conditional Knockout mouse — cardiac specific context
Beta myosin heavy chain is encoded by Myh7, ortholog of human MYH7, a slow ATPase motor whose head domain hydrolyzes ATP to drive actin filament sliding and whose rod mediates thick filament assembly, including the folded interacting heads motif that sets the super-relaxed state and therefore baseline energy consumption. Species context governs interpretation, since beta myosin dominates the human ventricle while the adult mouse ventricle runs mainly on alpha myosin from Myh6, so a mouse null is comparatively uninformative and reinduction of Myh7 is read as a marker of the fetal gene program in stress. Human missense variants cause hypertrophic and dilated cardiomyopathy through altered super-relaxed state occupancy, which is precisely the target of mavacamten, making patient-specific knockins or humanized alleles the correct instruments rather than deletion. Match alleles to endpoints including echocardiographic wall thickness, myofibrillar ATPase and loaded shortening, single-molecule motility, and myocardial disarray on histology.
Myh7 conditional knockout mice carry a floxed allele so you delete function only where Cre is active. The germline allele stays intact until you cross to a tissue specific Cre. Labs reach for this design when a global knockout is lethal, when they need adult onset loss, or when regional redundancy hides a whole body phenotype. For cardiac work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Catalog options
Conditional knockout focuses the experiment on heart while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
| Model | Type | Category | Availability | Catalog # | |
|---|---|---|---|---|---|
| Myh7-Flox | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 241457 | Inquire |
Designing a Myh7 Conditional Knockout allele
Adult cardiac deletion is nearly silent at baseline, since the adult mouse ventricle runs on alpha myosin, and that is precisely the experiment: remove the gene in adults, then impose transverse aortic constriction or pressure overload to ask whether stress reinduction of beta myosin is adaptive or contributes to decompensation. Use an inducible driver, ideally one not transcribed from the neighboring Myh6 promoter, or AAV9 delivered cTnT-Cre. The floxed design needs care because the Mhrt long noncoding RNA is transcribed antisense from this locus and shares its regulatory territory, so a large deletion can remove a chromatin-regulatory element rather than only the myosin. Skeletal muscle is the second application, using Acta1 or Ckmm drivers to strip slow type I fibers in soleus. Endpoints include isoform composition, soleus fiber typing, myofibrillar ATPase, and pressure-volume relations after loading.
Pricing and quotes
The Myh7 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Myh7 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with milestones for genotyping, QC, and dispatch.
FAQ
What Myh7 Conditional Knockout mouse models are available?
When Myh7 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Myh7 knockout embryonic lethal in mice?
It depends on background and allele design. Some Myh7 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for heart specific focused experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. Your note points to heart specific, so we start driver selection there.
Do you ship live Myh7 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Myh7?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.