Pfkp Knockout mouse models
Pfkp, the mouse ortholog of human PFKP, encodes the platelet-type phosphofructokinase 1 subunit, which assembles into tetramers with the L and M subunits and catalyzes the rate-committing conversion of fructose 6-phosphate to fructose 1,6-bisphosphate under allosteric control by ATP, citrate and fructose 2,6-bisphosphate. This isoform is the one most often upregulated in tumors and activated immune cells, where growth factor signaling through EGFR and AKT drives post-translational modification and membrane recruitment that raise glycolytic flux, and where ubiquitin-dependent degradation limits aerobic glycolysis. That biology makes conditional nulls the tool for asking whether tumor or immune cell glycolysis actually depends on this subunit in vivo, while humanization supports isoform-selective inhibitor work. Compensation by Pfkl and Pfkm within the shared tetramer pool is substantial, so plan compound conditional alleles rather than expecting a clean single-locus phenotype. Match the allele to lactate output, tumor growth or T cell effector function endpoints.
Global Pfkp deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Pfkp Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Pfkp knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Pfkp Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pfkp allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Pfkp Knockout mouse models are available?
When Pfkp Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pfkp knockout embryonic lethal in mice?
It depends on background and allele design. Some Pfkp germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Pfkp animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pfkp?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.