Pmaip1 Knockout mouse models
Pmaip1, the mouse ortholog of human PMAIP1, encodes NOXA, a short BH3-only protein induced transcriptionally by p53, HIF1A, and ATF4 during genotoxic, hypoxic, and integrated stress responses, and it binds selectively to MCL1 and A1 rather than to BCL2 or BCL-XL. By occupying these labile guardians and promoting MCL1 turnover through MULE-dependent ubiquitination, NOXA lowers the apoptotic threshold and liberates BAK and BAX for mitochondrial outer membrane permeabilization, which explains why NOXA levels predict sensitivity to proteasome inhibitors, CDK9 blockade, and venetoclax combinations. Conditional nulls therefore test survival requirements in a defined lineage, whereas knockins that ablate the BH3 helix or stabilize the protein against degradation distinguish loss of function from persistent priming. Redundancy is genuine, since Bbc3 (PUMA), Bcl2l11 (BIM), and Bid overlap, so compound alleles are often required. Anchor allele choice to thymocyte deletion, lymphoma latency, or BH3 mimetic pharmacodynamics.
A conventional Pmaip1 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Pmaip1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Pmaip1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Pmaip1-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 201813 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Pmaip1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pmaip1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Pmaip1 Knockout mouse models are available?
When Pmaip1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pmaip1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Pmaip1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Pmaip1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pmaip1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.