Prkag1 Knockout mouse models
Nucleotide sensing in AMPK resides in the gamma subunit, and Prkag1, the ortholog of human PRKAG1, encodes the ubiquitously expressed gamma1 isoform built from four cystathionine beta-synthase domains arranged as two Bateman modules that create four adenine nucleotide sites: one permanently occupied by AMP, one nonfunctional, and two that exchange AMP, ADP and ATP to convert energy charge into conformational change. AMP or ADP binding promotes Thr172 phosphorylation by LKB1, blocks its dephosphorylation, and allosterically stimulates catalysis, so gamma1 rather than the catalytic subunit sets the sensitivity threshold. Useful alleles include conditional nulls to test trimer availability and CBS-site knockins such as the activating R70Q substitution or nucleotide-binding-dead variants, which uncouple energy sensing from kinase competence in a way pharmacology cannot. Because gamma2 and gamma3 form functional trimers in heart and muscle respectively, interpret gamma1 loss against measured isoform stoichiometry in the target tissue.
A conventional Prkag1 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Prkag1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Prkag1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Prkag1-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 191110 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Prkag1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Prkag1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Prkag1 Knockout mouse models are available?
When Prkag1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Prkag1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Prkag1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Prkag1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Prkag1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.