Rad52 Knockout mouse models
Rad52, ortholog of human RAD52, encodes a single-stranded DNA annealing protein that assembles into undecameric rings, binds RPA-coated single-stranded DNA and catalyzes complementary strand annealing, driving single-strand annealing repair and break-induced replication as well as second-end capture during homologous recombination. Unlike its budding yeast counterpart it is not the principal RAD51 mediator in mammals, a role BRCA2 fulfills, and this is precisely why the mouse null is viable and nearly phenotypically silent with only modest recombination defects. That redundancy is the central design consideration: informative models place conditional or constitutive Rad52 loss on Brca1, Brca2 or Palb2 deficient backgrounds, where RAD52 becomes a synthetic lethal dependency and a validated small molecule target. Structure-guided knockins that disrupt the annealing groove or the RPA interaction while preserving ring assembly separate mechanism from protein abundance. Select by endpoint: compound conditional genetics for synthetic lethality, point knockins for target validation.
A conventional Rad52 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Rad52 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Rad52 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Rad52-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 240546 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Rad52 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Rad52 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Rad52 Knockout mouse models are available?
When Rad52 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Rad52 knockout embryonic lethal in mice?
It depends on background and allele design. Some Rad52 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Rad52 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Rad52?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.