Ros1 Knockout mouse models
Ros1 is the mouse ortholog of human ROS1 and encodes an orphan receptor tyrosine kinase, structurally related to Drosophila Sevenless, with an unusually large extracellular region of fibronectin type III and glycine-rich repeats and no validated mammalian ligand; physiologically it drives differentiation of the epididymal initial segment, and germline nulls are viable although males are infertile. Its clinical importance is entirely rearrangement driven, with CD74-ROS1, EZR-ROS1, SLC34A2-ROS1, and SDC4-ROS1 fusions retaining an intact kinase domain while the partner supplies dimerization or membrane anchoring, activating SHP2, RAS-ERK, PI3K, and STAT3 in lung adenocarcinoma, inflammatory myofibroblastic tumor, glioblastoma, and cholangiocarcinoma. Because there is no loss-of-function disease worth modeling, the informative alleles are inducible fusion knockins or humanized kinase domain replacements that support crizotinib, entrectinib, and repotrectinib pharmacology and reproduce G2032R solvent front resistance. Pair fusion expression with alveolar type II drivers such as Sftpc-creER for histologically appropriate lung tumors.
Global Ros1 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Ros1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Ros1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Ros1-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 240417 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Ros1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Ros1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Ros1 Knockout mouse models are available?
When Ros1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Ros1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Ros1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Ros1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Ros1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.