Srebf2 Knockout mouse models
Cholesterol homeostasis is governed largely by Srebf2, the ortholog of human SREBF2, whose SREBP-2 precursor resides in the endoplasmic reticulum bound to SCAP, is retained by INSIG when sterol levels are high, and upon sterol depletion travels to the Golgi for S1P and S2P cleavage that liberates a basic helix-loop-helix leucine zipper fragment driving Hmgcr, Hmgcs1, Ldlr and Pcsk9 transcription. The gene also hosts miR-33a within an intron, coordinating cholesterol synthesis with repression of Abca1-dependent efflux, a coupling that a naive coding knockout would disrupt inadvertently. Germline deletion is largely embryonic lethal, so hepatocyte or macrophage conditional nulls are the practical design, and intron-preserving strategies or separate miR-33a alleles are essential when efflux is an endpoint. Cleavage-resistant or constitutive nuclear-form knockins test whether sterol sensing rather than transcriptional capacity is limiting, and Srebf1 compensates only partially at shared sterol response elements.
Srebf2 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Srebf2 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Srebf2 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Srebf2-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 210188 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Srebf2 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Srebf2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Srebf2 Knockout mouse models are available?
When Srebf2 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Srebf2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Srebf2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Srebf2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Srebf2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.