Stat6 Conditional Knockout mouse models
Stat6, ortholog of human STAT6, encodes the transcription factor that converts type 2 cytokine input into gene expression, recruited by its SH2 domain to Jak-phosphorylated tyrosines on the shared Il4ra chain downstream of both the type I IL-4 receptor and the type II IL-4 and IL-13 receptor, then dimerizing and binding TTCNNNNGAA response elements to drive Gata3, Ccl26, Arg1 and immunoglobulin class switching to IgE. Because a single factor integrates two cytokines through one shared chain, a null collapses the entire axis and cannot distinguish IL-4 from IL-13 contributions, which is the principal design pitfall. Conditional deletion in T cells, B cells, macrophages or epithelium assigns allergic phenotypes to a compartment, while receptor-level alleles or blocking reagents separate the two ligands, and humanized alleles support dupilumab-class pharmacology directed at IL-4R alpha. Read out airway eosinophilia, serum IgE, alternative macrophage activation markers, and goblet cell metaplasia.
Conditional deletion of Stat6 limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to Stat6 floxed stock yields usable cohorts.
What Stat6 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Stat6 conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Stat6-Flox | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 2111257 | Inquire |
Designing a Stat6 Conditional Knockout allele
Design so the deleted allele cannot make an interfering product. Flanking the exons encoding the DNA binding domain removes target occupancy while leaving no species that still dimerizes through the SH2 domain and titrates partners. Useful drivers beyond the obvious lymphoid ones include Cd79a-Cre for early B cells, which deletes more completely than Cd19-Cre, Scgb1a1-CreERT2 for club cells and Foxj1-CreERT2 for ciliated cells when airway epithelial remodeling is the question, and Vil1-Cre for the intestinal tuft and goblet cell circuit driven by interleukin 25 and interleukin 13. The main pitfall is protein persistence, since STAT6 is long lived and recombination at the DNA level precedes functional loss by days. Confirm with phospho-STAT6 induction after interleukin 4 stimulation ex vivo. Endpoints are bronchoalveolar eosinophils, serum IgE, Retnla and Chil3 expression, and periodic acid Schiff staining.
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Stat6 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Stat6 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Stat6 Conditional Knockout mouse models are available?
When Stat6 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Stat6 knockout embryonic lethal in mice?
It depends on background and allele design. Some Stat6 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Stat6 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Stat6?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.