Stat6 Knockout mouse models
Stat6, ortholog of human STAT6, encodes the transcription factor that converts type 2 cytokine input into gene expression, recruited by its SH2 domain to Jak-phosphorylated tyrosines on the shared Il4ra chain downstream of both the type I IL-4 receptor and the type II IL-4 and IL-13 receptor, then dimerizing and binding TTCNNNNGAA response elements to drive Gata3, Ccl26, Arg1 and immunoglobulin class switching to IgE. Because a single factor integrates two cytokines through one shared chain, a null collapses the entire axis and cannot distinguish IL-4 from IL-13 contributions, which is the principal design pitfall. Conditional deletion in T cells, B cells, macrophages or epithelium assigns allergic phenotypes to a compartment, while receptor-level alleles or blocking reagents separate the two ligands, and humanized alleles support dupilumab-class pharmacology directed at IL-4R alpha. Read out airway eosinophilia, serum IgE, alternative macrophage activation markers, and goblet cell metaplasia.
A conventional Stat6 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Stat6 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Stat6 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Designing a Stat6 Knockout allele
The germline null is healthy at baseline, breeds normally, and is the cleanest available switch for the entire type 2 axis, which is exactly why it works better as a comparator than as an explanatory allele. Its most productive applications are compartment splitting experiments: bone marrow chimeras separating hematopoietic from stromal requirement, and transfer of wild type or deficient T cells into null hosts to ask whether STAT6 is needed in the responder or in the tissue. Background matters more than usual, since BALB/c animals carry a stronger baseline type 2 bias and give larger effect sizes in allergen models than C57BL/6. Delete the coding exons completely rather than an internal segment. Score Nippostrongylus brasiliensis expulsion kinetics and worm burden, airway hyperresponsiveness by forced oscillometry, serum IgE, Gata3 expression, and intestinal tuft cell frequency.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Stat6 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Stat6 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Stat6 Knockout mouse models are available?
When Stat6 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Stat6 knockout embryonic lethal in mice?
It depends on background and allele design. Some Stat6 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Stat6 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Stat6?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.