Traf6 Xenograft-Applicable mouse models
Encoding an essential K63-specific E3 ubiquitin ligase, Traf6 is the mouse ortholog of human TRAF6 and pairs its RING and zinc finger region with the UBC13 and UEV1A conjugating complex to build unanchored and substrate-linked K63 polyubiquitin chains that recruit TAB2 and TAB3 for TAK1 activation and NEMO for IKK activation, placing it immediately downstream of MyD88 and IRAK1 and IRAK4 for TLR and IL-1R signaling and downstream of RANK, CD40, and TGF-beta receptors. Its TRAF domain also autoubiquitinates and oligomerizes, giving threshold-like responses. Germline nulls die perinatally with severe osteopetrosis from failed osteoclast differentiation, absent lymph nodes, and neural tube defects, so conditional deletion is essential, while RING-dead or UBC13-binding knockins distinguish catalytic from scaffolding roles and are far more informative than a null for signaling questions. No paralog substitutes, so single-locus conditional genetics usually suffices. Align alleles to osteoclastogenesis, innate cytokine output, or regulatory T cell development.
Orthotopic and subcutaneous routes both benefit from consistent Traf6 genetics across cages.
What Traf6 Xenograft-Applicable mouse models are available?
ingenious targeting laboratory offers 1 distinct Traf6 xenograft-applicable catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Traf6-Flox/Lyz2-Cre(NMX) | Xenograft-Applicable | KO/CKO mice,Cre/Dre Toolbox of Mice | live | XA 252088 | Inquire |
Why this approach
We help teams pick alleles that match the experimental question, the organ system, and future breeding plans. For your target tissue centric work, Cre specificity, flox placement, and baseline controls matter as much as the gene edit itself.
Pricing and quotes
The Traf6 Xenograft-Applicable lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Traf6 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Traf6 Xenograft-Applicable mouse models are available?
When Traf6 Xenograft-Applicable lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Traf6 knockout embryonic lethal in mice?
It depends on background and allele design. Some Traf6 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Traf6 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Traf6?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.