stem cell specific Cre mouse lines for conditional alleles
Target conditional knockout mice carry a floxed allele so you delete function only where Cre is active. The germline allele stays intact until you cross to a tissue specific Cre. Labs reach for this design when a global knockout is lethal, when they need adult onset loss, or when regional redundancy hides a whole body phenotype. For stem cell work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Drivers used in stem cell programs
Conditional knockout focuses the experiment on stem-cell while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue. Knockin and humanized formats keep regulatory context at the endogenous locus. For stem-cell focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
- Lgr5-CreERT2
Inducible via tamoxifen control. Pair timing experiments with reporter crosses before scaling cohorts.
- Rosa26-CreERT2
Inducible via tamoxifen control. Pair timing experiments with reporter crosses before scaling cohorts.
Frequently asked questions
What does stem cell specific Cre mean?
stem cell specific Cre drivers recombine floxed alleles primarily in that lineage. Practical work still demands reporter crosses to verify efficiency in your facility because genetic background and copy number nudge leak profiles.
Should I use inducible CreERT2 for stem cell studies?
Inducible systems help when developmental deletion confounds adult phenotypes or when you need tight timing around injury or tumor onset. Tamoxifen protocols carry their own controls, which we document in project planning.
Where do I find floxed models to pair with these drivers?
Our catalog lists floxed conditional lines by gene. If your favorite target is not listed, we quote generated flox builds and crossing plans so you reach cohort size on a clear schedule.