Cd274 Mouse Models

Why Model Cd274

Cd274 is the mouse ortholog of human CD274 and encodes PD-L1 (B7-H1), a cell-surface ligand expressed on various immune, epithelial, and tumor cells. PD-L1 binds the inhibitory receptor PD-1 and has been associated with downregulation of T-cell responses.

PD-L1 expression on tumor cells has been associated with immune evasion in some contexts, and antibodies targeting PD-L1 are approved for several cancers; responses depend on tumor type and other factors. Cd274-deficient mice are viable and show altered T-cell responses in disease models.

Model Applications

Humanized CD274 models, in which the human ligand replaces the mouse counterpart, have been used to evaluate anti-PD-L1 antibody therapeutics in immunocompetent mice, including in combination with other checkpoint-targeted agents.

Cd274 knockout mice have been used to study the ligand’s role in immune regulation.

Custom Cd274 Model Options

Engineered to your specifications, with molecular validation of the targeted allele and germline transmission guaranteed.

• Conventional knockout
• Conditional knockout
• Point mutation / knockin
• Reporter knockin
• Humanized
• Targeted transgenic

Sources

• PD-1/PD-L1 immune checkpoint blockade in breast cancer: research insights and therapeutic strategies. Mol Cancer. 2024. doi:10.1186/s12943-024-02176-8
• Freeman GJ et al. Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med. 2000. doi:10.1084/jem.192.7.1027
• Dong H et al. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat Med. 1999. doi:10.1038/70932

Last reviewed: October 22, 2026 · Reviewed by: Paul Sheiffele

Related gene targets: Pdcd1 · Pdcd1lg2 · Cd80 · Cd86