Skip to main content
Back to Glossary
Recombination Systems & Tools

Flp-FRT System

A yeast-derived site-specific recombination mechanism enabling targeted DNA excision, inversion, or integration through the interaction of Flp recombinase with FRT sites. It serves as an orthogonal complement to Cre-lox in mammalian genetics.

Overview

The Flp-FRT system is a yeast-derived site-specific recombination mechanism enabling targeted DNA excision, inversion, or integration through the interaction of Flp recombinase with FRT sites. It serves as an orthogonal complement to Cre-lox in mammalian genetics.

Frequently Asked Questions

What is FlpO?

A thermostable Flp recombinase variant optimized for efficient activity in mammalian systems at 37°C.

When to use Flp-FRT vs Cre-lox?

Use Flp-FRT for cassette removal, dual-recombinase logic, or intersectional experiments requiring non-overlapping target sites.

Related Services

Related Terms

Cre-lox System

A bacteriophage P1-derived site-specific recombination technology that utilizes Cre recombinase to mediate genomic modifications at engineered LoxP sequences. This technology underpins conditional gene engineering approaches in mammalian genetics.

Dre-rox System (Alternate Recombinase)

An independent site-specific recombination platform derived from bacteriophage D6, using Dre recombinase to recognize rox sites for DNA rearrangements orthogonal to both Cre-lox and Flp-FRT.

Selection Markers (NeoR, PuroR)

Genes incorporated into targeting vectors to identify and isolate cells that have successfully integrated a genetic modification. Common markers include neomycin resistance (NeoR) and puromycin resistance (PuroR).

More in Recombination Systems & Tools

Cre-lox SystemCre Driver Line (Tissue-Specific Cre)Floxed Gene / LoxP SiteLox-STOP-Lox (LSL) CassetteDre-rox System (Alternate Recombinase)
Floxed Gene / LoxP SiteView All TermsFrameshift Mutation

Need Help with Your Mouse Model Project?

Our scientific consultants can help you understand the best approach for your research goals.

Request a QuoteContact Us